細(xì)胞簡介:（規(guī)格：KB） | Growth Properties: | adherent | | Organism: | Homo sapiens （human） | | Morphology: | epithelial | | Comments: | This line was originally thought to be derived from an epidermal carcinoma of the mouth, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
The cells are positive for keratin by immunoperoxidase staining.
KB cells have been reported to contain human papillomavirus 18 （HPV-18） sequences. | | Propagation: | Complete growth medium: RPMI-1640+10%CS+P/S Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide （CO2）, 5% | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium renewal: 2 to 3 times per week | | Preservation: | Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature | 常見問題及解決方案: 1、培養(yǎng)瓶有破裂，培養(yǎng)液有漏液：細(xì)胞極大可能會(huì)污染，所以我們會(huì)及時(shí)安排幫老師解決。 2、 細(xì)胞漂?。号囵B(yǎng)瓶不開封，瓶口酒精擦拭后平躺放置在培養(yǎng)箱。次日觀察，如細(xì)胞大部分又貼回瓶底，表明細(xì)胞活力正常，剩余漂浮的細(xì)胞可以離心去掉，留10ml培養(yǎng)液培養(yǎng)觀察，細(xì)胞生長至匯合度80%，進(jìn)行消化傳代；如細(xì)胞還是不貼壁，將細(xì)胞離心收集轉(zhuǎn)到新培養(yǎng)瓶，原培養(yǎng)瓶加部分培養(yǎng)液繼續(xù)培養(yǎng)，中間注意觀察，我們的技術(shù)人員會(huì)一直跟蹤指導(dǎo)，直到問題解決。 客戶收到細(xì)胞后請務(wù)必仔細(xì)閱讀細(xì)胞注意事項(xiàng)，確保細(xì)胞的培養(yǎng)條件*，如果由于培養(yǎng)條件不*導(dǎo)致細(xì)胞出現(xiàn)問題，責(zé)任由客戶自行承擔(dān)。由于運(yùn)輸?shù)那闆r，所以極個(gè)別細(xì)胞會(huì)出現(xiàn)不穩(wěn)定，客戶收到細(xì)胞后務(wù)必*時(shí)間和我們，告知細(xì)胞具體情況，以便我們技術(shù)人員能及時(shí)有效的和老師溝通，不勝感謝！ |
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